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. 2021 Dec 9;21:658. doi: 10.1186/s12935-021-02355-3

Fig. 4.

Fig. 4

Inhibitory effect of PG on CCA cell activity is mediated by SNAREs complex. AE HuCCT-1 and TFK-1 cells were infected with overexpressed STX17 and SNAP29 adenovirus, respectively. Cells were evaluated as follows: A Cell viability with CCK-8 after the addition of DMSO or PG. B Clone formation test to determine the rate of clone formation. C EDU test to detect cell proliferation. D Apoptosis of HuCCT-1 and TFK-1 cells by flow cytometry. E Western blot assay was used to detect the expression of apoptosis-related proteins; *P < 0.05