. 2021 Dec 10;525:46–53. doi: 10.1016/j.cca.2021.12.004

Table 1.

Basic information on five commercial RT-qPCR diagnostic test kits for SARS-CoV-2 evaluated in this study.

Kit	Gene	Template quantity/μl	Reaction system/μl	Number of cycles/Pieces	PCR reaction time (min)	Internal standard type	Declaration of detection limit (copies/ml)	Results interpretation criteria
Kit	Gene	Template quantity/μl	Reaction system/μl	Number of cycles/Pieces	PCR reaction time (min)	Internal standard type	Declaration of detection limit (copies/ml)	Positive	Suspicious	Negative
Da An	ORF1ab, N	5	25	45	118	Endogenous	500	Ct ≤ 40		Ct > 40
Liferiver	ORF1ab, N, E	5	25	45	90	Exogenous	1,000	Ct ≤ 43		Ct > 43
Kinghawk	ORF1ab, N	5	25	40	98	Endogenous	500	Ct < 38	38 < Ct < 40	No Ct
eDiagnosis	ORF1ab, N	5	25	40	82	Endogenous	500	Ct < 38	38 ≤ Ct < 40	Ct ≥ 40
Maccura	ORF1ab, N, E	20	40	40	88	Exogenous	1,000	Ct ≤ 38		Ct > 38 or No Ct