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. 2021 Dec 10;25(1):103589. doi: 10.1016/j.isci.2021.103589

Figure 3.

Figure 3

SARS-CoV-2 B.1.1.7 variant infectivity ex vivo

(A) SARS-CoV-2 WA1/2020 and B.1.1.7 growth curves on Vero E6 and Vero-TMPRSS2 cells. Cells were infected to an MOI of 0.1 and supernatant was collected at 0, 6, 12, 24, and 48 h p.i. Growth curves were then calculated using TCID50. Error bars represent ± standard deviation (n = 3).

(B). Immunofluorescence assay (IFA) of SARS-CoV-2 WA1/2020 and B.1.1.7 infection on Vero E6 and Vero-TMPRSS2 cells. Cells were infected to an MOI of 0.1 and IFA was performed 0, 6, 12, 24, and 48 h p.i. Detection of infected cells (green) was conducted using an antibody targeting the SARS-CoV-2 S2 domain. Cell nuclei were stained using DAPI (blue).