Figure 5.

Infection in respiratory Calu-3 cells

(A) Pseudoparticle infectivity assays in Calu-3 cells. Cells were infected with MLV pseudoparticles harboring the VSV-G, SARS-CoV-2 S WT, SARS-CoV-2 S B.1.1.7 variant, SARS-CoV-2 S WT with P681H mutation. Data represent the average luciferase activity of cells of three biological replicates. A significant increase in the luciferase transduction was observed with the pseudoparticles harboring the B.1.1.7 S (P = 0.0076). Error bars represent ± standard deviation (n = 3). Asterisks indicate statistical significance compared to the untreated control. Statistical analysis was performed using an unpaired Student’s t test. ∗∗ p < 0.01.

(B) SARS-CoV-2 WA1/2020 and B.1.1.7 growth curves on Calu-3 cells. Cells were infected to an MOI of 0.1 and supernatant was collected at 0, 6, 12, 24, and 48 h p.i. Growth curves were then calculated using TCID₅₀. Error bars represent ± standard deviation (n = 3).