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. 2021 Nov 26;9:760730. doi: 10.3389/fbioe.2021.760730

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Copyright © 2021 Stanly, Kim, Antonucci, Fiume, Guescini, Kim, Ciardiello, Giangrieco, Mari and Pocsfalvi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Nanoparticle tracking analysis showing the size distribution of microvesicles (MVs), nanovesicles (NVs), and the sucrose density gradient purified nanovesicle fraction (DGUC-NVs). Particle numbers, mean diameter values, and standard deviations are reported in Table 1. (B) The pictures on the left show the sample of strawberry-derived NVs after iodixanol density gradient ultracentrifugation (DGUC). After DGUC, a single visible band could be observed (orange arrow). The densities measured in the 12 different fractions from top to bottom are shown in the chart on the right. The visible band was observed in fraction 9. The vesicles floating in fraction nine are similar in density to mammalian extracellular vesicles, which have a density of 1.13–1.19 g/ ml (Konoshenko et al., 2018).