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Sensitivity of the PcSA1 RT-PCR. RT-PCRs were carried out on total RNA preparations prepared from log-unit dilutions of viable P. carinii. Lane 6, 106 P. carinii organisms; lane 5, 105 P. carinii organisms; lane 4, 104 P. carinii organisms; lane 3, 103 P. carinii organisms; lane 2, 102 P. carinii organisms; lane 1, 101 P. carinii organisms; lane −RT, without reverse transcriptase (RNA from 106 organisms); lane −, PCR negative control.
