FIG. 4.

Species specificity of the PcSA1 RT-PCR. RT-PCRs were carried out on fungal RNA preparations prepared as described in Materials and Methods. (A) EB-stained gel of PCR products; (B) Southern blot of the gel shown in panel A probed with the labeled PcSA1 PCR product. In each panel, lane 1 contains RNA extracted from P. carinii, lane 2 contains RNA extracted from S. cerevisiae, lane 3 contains RNA extracted from Penicillium chrysogenum, and lane 4 contains RNA extracted from A. niger.