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. 2021 Dec 8;9(12):e002772. doi: 10.1136/jitc-2021-002772

Figure 5.

Figure 5

Differentially expressed gene sets (GO category) and selected genes analyzed by RNA SEQ related to the immune response in agents (DMSO + IgG or SN-38 + PD-1 Ab) treated tumor tissues. (A) Top 10 biological functional annotated increased or decreased gene expression were performed in DAVID (web-based tool). Each bar presents the number of genes in signature categories. (B) The composition of immune cell types was predicted by the EPIC web-based tool. The SN-38 + PD-1 Ab treated tumor samples predicted more T cells and NK cells in the tumor tissues. (C) This panel shows T cell or NK cell markers’ differential expression, transcripts per million (TPM). The tumor samples treated with SN-38 plus PD-1 Ab showed increased T cell and NK cell markers expression. (D) The representative color heat maps based on Z-score significantly increased genes related to T cell or NK cell activation (D: DMSO + IgG treatment, S: SN-38 + PD-1 Ab treatment). Ab, antibody; DAVID, Database for Annotation, Visualization, and Integrated Discovery; DMSO, dimethylsulfoxide; EPIC, Estimating the Proportions of Immune and Cancer; GO, Gene Ontology; IFN-γ, interferon-γ; NK, natural killer; PD-1, programmed death-1.