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. Author manuscript; available in PMC: 2022 Feb 15.
Published in final edited form as: J Immunol. 2021 Jan 11;206(4):766–775. doi: 10.4049/jimmunol.2000591

Figure 5. IL-17A induces TNFSF13 expression in human and mouse colonic organoid cultures and overexpression of Tnfsf13 increases IgA+ cells.

Figure 5.

(A) Gene expression of Pigr, Tnfsf13 and Tnfsf13b in mouse colonic organoids as measured by RT-PCR. (n=4). (B) Left: Human TNFSF13 concentrations in human organoid culture medium was measured by ELISA. Right: TNSF13 mRNA expression in human colonic organoids measured by RT-PCR. (C-D) Effect of Tnfsf13 overexpression on Ig+ cells. Il17ra fl/fl villin cre+ and littermate cre- mice were intraperitoneally injected with Ad-Tnfsf13 or Ad-zsGreen at day3 post infection of C. rodentium and followed for 14 days. (C) Right: C. rodentium RNA expression in colon measured by RT-PCR, Left: bacteria burdens in colon (CFU). (D) C. rodentium specific IgA secreting cells in spleen were measured by Elispot. Significant differences are designated by 2-tailed Student’s t test (B). Significant differences are designated by using ANOVA followed by Tukey’s multiple comparisons test (A and D). *, P < 0.05; **, P < 0.01. Values are means ± SEM.