Fig. 6.
Evaluation of cartilage formation of trachea-mimetic cellular construct in vivo. (A, B) The H&E, (C, D) Alcian Blue, (E, F) Safranin-O, and (G, H) Masson’s Trichrome staining result of longitudinal cross-section of cartilage rings from retrieved cellular constructs with (left) and without (right) wrapping of SPTM after 8 weeks of implantation. Scale bars, 500 μm. The immunofluorescence image of (I) COL2 and (J) ACAN in the retrieved trachea-mimetic cellular tracheal construct with wrapping of SPTM after 8 weeks of implantation. Scale bars, 200 μm.