Figure 1.

Increased neutrophil extracellular traps and arterial thrombosis in Lnk–/– mice are reversed by PAD4 deficiency. A, FeCl₃-induced carotid artery occlusion in wild-type (WT), Lnk^–/–, Pad4^–/–, and Lnk^–/– Pad4^–/– bone marrow female Ldlr^–/– recipient mice fed Western-type diet for 10 weeks; 2-way analysis of variance. B, Time to occlusion measured in WT and Lnk^–/– bone marrow recipient mice after 48 hours of treatment with 100 µg anti-Ly6G or isotype control antibody; 2-way analysis of variance. C, Representative images of Carstairs, fibrinogen (red), or neutrophil extracellular trap (NET) staining in carotid artery thrombi of WT, Lnk^–/–, Pad4^–/–, and Lnk^–/–Pad4^–/– bone marrow female Ldlr^–/– recipients fed Western-type diet for 10 weeks. NETs were validated using MPO (green) with citrullinated histone (H3Cit; red) or MPO (green) with histone 3 (red). NETs shown were from the same area as in fibrinogen staining on consecutive sections. Quantification of NETs (n=4 per group); 2-way analysis of variance. Each point represents 1 mouse. Scale bar, 50 µm. Data are expressed as mean (red bar) ±SEM. IgG indicates immunoglobulin G.