Table 1. Results of PCR-AGE and RPA-LF methods for detection of E. faecium, vanA and vanB genes.

Organisms (n)	Number of positive result
	E. faecium			vanA			vanB
	PCR-AGE a	RPA-LF b	RPA-LF c	PCR-AGE a	RPA-LF b	RPA-LF c	PCR-AGE a	RPA-LF d
Bacterial isolates (126)
E. faecium (20)	20	20	20	0	0	0	0	0/6
vanA-carrying E. faecium (35)	35	35	35	35	35	35	0	0/35
vanB-carrying E. faecium (1)	1	1	1	0	0	0	1	1/1
E. faecalis (48)	0	0	0	0	0	0	0	0/5
vanB-carrying E. faecalis (1)	0	0	0	0	0	0	1	1/1
E. gallinarum (2)	0	0	0	0	0	0	0	0/2
E. casseliflavus (1)	0	0	0	0	0	0	0	0/1
E. raffinosus (1)	0	0	0	0	0	0	0	0/1
E. avium (1)	0	0	0	0	0	0	0	0/1
S. agalactiae (5)	0	0	0	0	0	0	0	ND
S. pyogenes (3)	0	0	0	0	0	0	0	0/1
S. dysgalactiae (1)	0	0	0	0	0	0	0	ND
S. constellatus (1)	0	0	0	0	0	0	0	ND
S. aureus (1)	0	0	0	0	0	0	0	0/1
S. haemolyticus (1)	0	0	0	0	0	0	0	ND
E. coli (1)	0	0	0	0	0	0	0	0/1
K. pneumoniae (1)	0	0	0	0	0	0	0	0/1
A. baumannii (1)	0	0	0	0	0	0	0	0/1
P. aeruginosa (1)	0	0	0	0	0	0	0	0/1
Positive blood culture (15)
E. faecium (4)	4	4	ND	0	0	ND	0	0/4
E. faecalis (5)	0	0	ND	0	0	ND	0	0/5
E. casseliflavus (1)	0	0	ND	0	0	ND	0	0/1
S. aureus (1)	0	0	ND	0	0	ND	0	0/1
S. epidermidis (1)	0	0	ND	0	0	ND	0	0/1
Coagulase negative staphylococci (1)	0	0	ND	0	0	ND	0	0/1
S. viridans (1)	0	0	ND	0	0	ND	0	0/1
S. gallolyticus (1)	0	0	ND	0	0	ND	0	0/1
Rectal swab (10)
E. faecium (6)	6	ND	6	0	ND	0	ND	ND
vanA-carrying E. faecium (1)	1	ND	1	1	ND	1	ND	ND
E. faecalis (1)	0	ND	0	0	ND	0	ND	ND
group D streptococci non-enterococcus (2)	0	ND	0	0	ND	0	ND	ND
Total (151)	67	60	63	36	35	36	2	2/74

Notes.

ND

not done

^aThe conventional PCR method was tested using DNA from bacterial colonies.

^bRPA-LF method for E. faecium and vanA was tested in positive blood culture samples.

^cRPA-LF method for E. faecium and vanA was tested in stool/rectal swab samples.

^dRPA-LF method for vanB was tested in 74 positive blood culture samples.