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. 2021 Nov 22;6(22):e125635. doi: 10.1172/jci.insight.125635

Figure 4. BLM-induced SREBP2 suppresses EC markers.

Figure 4

(A) HUVECs were treated with BLM (1 mU) for 72 hours. (B) HUVECs were infected with Ad-SREBP2(N) or empty vector (Ad-Null) for 72 hours. The mRNA expression levels of VE-Cadherin (VE-Cad), kinase insert domain receptor (KDR), and Krüppel-like factor 2 (KLF2) were measured using qPCR. (C) ATAC-Seq indicates the chromatin state of genes specific to EC markers upon SREBP2(N) overexpression. (D) H3K27ac ChIP-qPCR showing the chromatin state of indicated genes following 72 hours of Ad-SREBP2(N) infection compared with empty vector. (E) HUVECs were treated with the indicated concentration of BLM. DNA methyltransferase 1 (DNMT1) activity was measured using ELISA. (F and G) HUVECs were treated with BLM or infected with Ad-SREBP2(N) or Ad-Null for 72 hours. Isolated DNA was bisulfite converted and subjected to methylation-specific qPCR. Data in A, B, and D–G were analyzed by 2-tailed Student’s t test; data are represented as mean ± SEM from 3 independent experiments (n = 3). *P < 0.05 between the indicated groups.