Figure 5. Peritransplant glucocorticoid treatment enhances GVL.
(A) B6D2F1 mice were transplanted with 5 × 106 BM, 0.25 × 106 T cells, and 1 × 106 BCR/ABL-NUP98/HOXA9 (GFP+) leukemia cells to compare GC treatment with PT-Cy conditioning (50 mg/kg, days +3 and +4). (B) Representative flow analysis of peripheral blood showed GFP+ leukemia cells at day 28 after transplant. (C) Leukemia cell numbers in the peripheral blood of each group compared by Student’s t test at the indicated time points. *P < 0.05; **P < 0.01. (D) Proportions of leukemia death. GC-treated versus vehicle-treated BMT recipients at day 70. **P = 0.0052 by log-rank analysis. Results are combined from 3 experiments with 10–20 mice per group.