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. 2021 Nov 22;6(22):e150999. doi: 10.1172/jci.insight.150999

Figure 1. Circulating B cells in patients with PR3-AAV and MPO-AAV and HCs.

Figure 1

Gating strategy used to define B cell subsets (A). CD19+ cells were first categorized based on CD24 and CD38 expression, in transitional B cells (transi, CD24hiCD38hi), plasmablasts (PB, CD24–CD38hi), and mature B cells (CD24+CD38+). Mature B cells were further classified into 4 populations: naive (CD27–IgD+), unswitched memory (UnSW; CD27+IgD+), switched memory (SW; CD27+IgD–), and double negative (DN, CD27–IgD–). B cell frequency and subset distribution were overall similar in patients with PR3-AAV (n = 105) and MPO-AAV (n = 49) but different compared with HCs (n = 27) (B and C). Principal component analysis of the 200 B cell clusters obtained with spanning-tree progression analysis of density-normalized events (SPADE) representing HCs and PR3-AAV trial participants (D) and participants with MPO-AAV and PR3-AAV (E). Data represent median (25%–75% IQR). Multiple comparisons among more than 2 groups were performed with Kruskal-Wallis test. *P < 0.05, **P < 0.01, ***P < 0.001 after correction for FDR with Benjamini and Hochberg test.