Figure 4. Semaglutide reduces liver fibrosis and hepatic cytokine gene expression in Glp1r^Tie2+/+ mice but not in Glp1r^Tie2–/– mice.

Analysis of liver tissue from PCSK9+HFHC-fed mice treated with vehicle (Veh) or semaglutide (Sema) for 18 weeks: (A) Col1a1 versus Ppia expression, (B) Picrosirius red⁺ (PSR⁺) collagen staining quantification (n = 5), and (C) representative images (scale bar: 500 μm). (D) Liver tissue triglycerides (TG), (E) liver expression of Glp1r and metabolic regulators, and (F) inflammation markers versus Ppia (n = 6–14). Data are presented as mean ± SD, with individual data points shown. *P < 0.05 for Sema effect, 2-way ANOVA multiple-comparison test; ^#P < 0.05, 2-way ANOVA effect for treatment; ‡P < 0.05, 2-way ANOVA effect for genotype.