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. 2021 Nov 22;6(22):e152014. doi: 10.1172/jci.insight.152014

Figure 4. Polyfunctional responses in CD4+ and CD8+ CAR T cells in response to antigen.

Figure 4

CD4 and CD8 subsets of untransduced or GD3 CAR transduced T cells were magnetically separated and individually cocultured 2:1 with GD3-expressing HEK293 cells for 20 hours before loading onto IsoCode chips. (A) Wells containing single live cells were analyzed by IsoSpeak data visualization software, and detection of individual analytes is represented by signal intensity per cell. Horizontal bars denote the mean for each analyte. (B) The percentages of single T cells secreting the indicated number of cytokines are shown in pie charts, with the percentage of polyfunctional T cells (expressing 2 or more cytokines) listed in the center of the pie.