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. 2001 Jan;21(2):644–654. doi: 10.1128/MCB.21.2.644-654.2001

FIG. 2.

FIG. 2

β-Galactosidase expression. (A) Expression of NRF-1 and the NRF-1–β-galactosidase fusion gene transcripts in adult mice. A total of 10 μg of RNA isolated from the indicated tissues of an NRF-1+/− mouse (lanes 1 to 6) or from kidney of a wild-type littermate (lane 7) was analyzed for the expression of NRF-1 and NRF-1–β-galactosidase transcripts by RNase protection assay. Lane 6, with the NRF-1–β-galactosidase riboprobe alone; other lanes, with both NRF-1 and NRF-1–β-galactosidase riboprobe. The sizes of the protected products are indicated on the right. (B) β-Galactosidase expression in embryos. Embryonic stages are indicated on the left, and the various crosses are shown above. F, female; M, male. Positively stained embryos are indicated by arrows. β-Galactosidase activity was readily detected in eight-cell morulae (b and c) and in both the inner cell mass and trophectoderm cells in blastocysts (e and f).