NRF-1 expression in ovary. (A) β-Galactosidase staining of an ovary from a wild-type mouse. (B) β-Galactosidase staining of an ovary from a NRF-1+/− mouse. (C to F) β-Galactosidase staining of follicles from a NRF-1+/− mouse containing eggs at various stages of maturity. The corpus luteum (CL), the thecal cell layer surrounding the follicle (T), the follicle (Fl), the zona pellucida (ZP, unstained), the cytoplasm (Cy), and the nucleus (Nu) are indicated (B and E). The concentrated regions of β-galactosidase accumulation within the cytoplasm are indicated by arrows in panels C and F. Panels A and B are at the same magnification; panels C to F are at the same magnification.