Table 2.

PRP method of preparation of included studies

Author	Study design	Sample size	Volume of blood drawn	PRP volume	Activator	Anticoagulant	Method of PRP preparation (any special features)
PRP monotherapy for facial rejuvenation
Sevilla et al.[8]	Single blind, split-face prospective, comparative cohort	80 (1:20) (2:60)	34 ml	2.5 ml	nil	Acid citrate dextrose	Double spin 1): 150 g×15 min 2): 2700 rpm×10 min
Alam et al.[9]	Double-blind, split-face RCT	19	20 ml	3 ml	Nil	Acid citrate dextrose	Smart PREP2 APC (Harvest technologies)
Zenker[10]	Case series	418 from Germany, Japan, UK, Israel	10 ml	4 ml	Nil	NA	Single centrifugation
PRP in combination with laser resurfacing
Hui et al.[11]	Single- blind split-face RCT	13	30 ml	2.2 ml	CaG	Heparin calcium	Double spin 1) 1200 rpm for 10 min 2) 3500 rpm for 5 min
Shin et al.[12]	Randomized controlled study, single-blind	22	12 ml	3 ml	CaCI2	CPD- A	Double spin 3000 rpm/5 min
PRP in combination with lipofilling
Willemsen et al.[13]	Double-blind, RCT	25 (1:13) (2:12)	30 ml	3 ml	Nil	Acid citrate dextrose	3000 rpm/15 min
PRP in treatment of acne scarring
Chawla[14]	Case series	30	10 ml	NA	Calcium gluconate	Acid citrate dextrose (ACD)	Double spin 1) 1500 rpm for 10 min 2) 3700 rpm for 10 min
Nofal et al.[15]	Randomized controlled trial	45	10 ml	2 ml	CaCI2	NA	Double spin 1) 175 g for 10 min 2) 1750 g for 15 min
Babu et al.[16]	Case series	20	10 ml	NA	NA	Acid citrate dextrose (ACD)	Two stage 1) 1500 rpm for 10 min 2) 3000 rpm for 20 min
PRP for infraorbital rejuvenation
Kang et al.[17]	Single blind Split-face RCT	16	12 ml	1 ml	CaCI2	Acid citrate dextrose	My cells kit (Estar technology Ltd)
Mehryan et al.[18]	Case series	10	10 ml	1.5 ml	CaCI2	Acid citrate dextrose	PRP kit (Kimia teb rahavard co)