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. 2021 Dec 10;16(12):e0251995. doi: 10.1371/journal.pone.0251995

Fig 2. Tmprss6-ASO reduced erythropoiesis in the bone marrow of PV mice.

Fig 2

Flow cytometry analysis of bone marrow and spleen (A) showed signs of iron restricted erythropoiesis as indicated by decreased erythroblast maturation with a decreased ratio of enucleated:nucleated (E:N) cells, consistent with more ineffective erythropoiesis. Flow cytometry analysis of the five clusters of erythroid populations shows significant evidence of decreased erythroblast differentiation in the BM (orthochromatic erythroblasts /reticulocytes, and mature red cells) (B), while only a trend can be appreciated in the spleen (mature red cells) (C). For these analyses were used BMT-WT mice (n = 3), BMT-PV-CTRL-ASO mice (n = 6), and BMT-PV-Tmprss6-ASO mice (n = 7). Results represent mean ± SD. Bars represent standard deviation (SD). 2-way ANOVA (with Tukey’s multiple comparisons tests) was used for the statistical analysis. Asterisks refer to statistically significant differences: ****P≤ 0.001, ***P≤ 0.005. Only statistical differences between BMT-PV-Ctrl-ASO and BMT-PV-Tmprss6-ASO mice are indicated in the graphs.