TABLE 1.
Summary of the Cas13 effectors and their applications for viral RNA degradation and CRISPR-based diagnostics.
| Application | Viruses | Cas13 orthologs | References |
| A. RNA degradation | |||
| Turnip mosaic virus (TuMV) | LshCas13a | Aman et al., 2018a,b | |
| Turnip mosaic virus (TuMV) Tobacco mosaic virus (TMV)-RNA-based overexpression (TRBO-G) | LwaCas13a PspCas13b CasRx | Mahas et al., 2019 | |
| Potato virus Y (PVY) | LshCas13a | Zhan et al., 2019 | |
| Southern rice black-streaked dwarf virus (SRBSDV) | LshCas13a | Zhang et al., 2019 | |
| Influenza A virus (IAV) Respiratory syncytial virus model (RSV) | LbuCas13a | Bawage et al., 2018 | |
| Lymphocytic choriomeningitis (LCMV) Vesicular stomatitis virus (VSV) IAV | LwaCas13a PspCas13b | Freije et al., 2019 | |
| Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) IAV | CasRx | Abbott et al., 2020 | |
| SARS-CoV-2 IAV | LbuCas13a | Blanchard et al., 2021 | |
| Hepatitis C virus HCV | LshCas13a | Ashraf et al., 2021 | |
| Porcine reproductive and respiratory syndrome virus (PRRSV) | PspCas13b | Cui et al., 2020 | |
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| B. CRISPR-based diagnosis | |||
| ZIKA and Dengue RNA (SHERLOCK) | LwaCas13a | Gootenberg et al., 2017 | |
| ZIKA and Dengue RNA (SHERLOCKv2) | LwaCas13a, CcaCas13b LbaCas13a PsmCas13b | Gootenberg et al., 2018 | |
| Flaviviruses (HUDSON-SHERLOCK) | LwaCas13a | Myhrvold et al., 2018 | |
| All 169 human-associated viruses, Including IAV, SARS-CoV-2, HIV (CRISPR-Cas13 with CARMEN) | LwaCas13a | Ackerman et al., 2020 | |
| Ebola virus (EBV) and Lassa virus (SHERLOCK-HUDSON) | LwaCas13a | Qin et al., 2019; Barnes et al., 2020 | |