Fig. 2. Effects of NS on the in vitro formation of amyloid by Aβ^1-42, ccβω, and α-syn.

Plots of ThioT fluorescence [arbitrary fluorescence units (AFU)] as a function of time for aggregation reactions containing (A) Aβ^1-42, (B) ccβω, and (C) α-syn. The aggregation reactions for Aβ^1-42 and ccβω were unseeded but that for α-syn was seeded (see Materials and Methods). Nonchaperone control proteins (SOD, OVA, and BSA) and the molar ratio of these or NS to the aggregating protein are indicated in the respective keys. When incubated alone under these conditions, NS did not generate any significant ThioT fluorescence (fig. S1). Data points plotted are means ± SEM (n = 3), and each result shown is representative of two to three independent experiments.