Fig. 7. Effects of mutations in the conserved 14-mer region on the ability of NS to inhibit in vitro amyloid formation.

ThioT fluorescence (AFU) was used to monitor the formation of amyloid by (A) Aβ^1-42 and (B) ccβω. The molar ratio of nonchaperone control proteins (SOD and α-lac) or NS^wt/NS^sm/NS^tm to the aggregating protein was, in all cases, 1:10. Data plotted are means ± SEM (n = 3). In some cases, the error bars are too small to be visible. Each result shown is representative of two independent experiments.