Fig. 8. Effects of mutations in the conserved 14-mer region on the ability of NS to inhibit amyloid cytotoxicity.

Samples taken from aggregation mixtures of Aβ^1-42 and ccβω were incubated with (or without) NS^wt/NS^sm/NS^tm before adding to NSC34 cells; cell viability was later quantified using calcein-AM as described in Materials and Methods. In all cases, the molar ratio of NS:Aβ^1-42 or NS:ccβω was 1:10. Results are presented as percent cytotoxicity relative to Aβ^1-42 or ccβω alone. Under the conditions tested, relative to the control, the percentage of viable cells for cultures treated with Aβ^1-42 or ccβω alone were 45 and 31%, respectively. Values plotted represent means ± SEM (n = 16). Statistically significant differences have been indicated by an asterisk [one-way analysis of variance (ANOVA), P < 0.05]. The results shown are each representative of two independent experiments.