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. 2021 Dec 10;7(50):eabj7857. doi: 10.1126/sciadv.abj7857

Fig. 8. Newly bone formation in a rat cranial bone defect model.

Fig. 8.

(A) μCT images of calvaria samples, (A, a to d) samples from 2-week post-surgery group. (A, e to h) Samples from 8-week post-surgery group. Images of (A) (a to h) exhibit the same scale bar of 1 mm. (B) Quantitative analysis of newly bone formation at 2 and 8 weeks. Notes: Bone volume/tissue volume (BV/TV), bone marrow density (BMD), trabecular thickness (Tb.Th), and structure model index (SMI). *P < 0.05, **P < 0.01. (C) Representative sequential fluorescent staining for alizarin red, tetracycline, and calcein at 1, 3, and 5 weeks. (C, a to p) Images share the same scale bar of 250 μm. (D) H&E and Masson’s trichrome staining of samples at 8 weeks, H&E staining was shown in (D) (a, c, e, and g) and Masson’s trichrome staining in (D) (b, d, f, and h). Images of (D) (a to h) exhibit the same scale bar of 1 mm. Black arrows in the figure suggest the mineralized zone.