Fig. 7. E-box;GATA and Ets motif variants attenuate hematopoietic regenerative response to inflammation.

(A) Flow cytometry scheme for quantification of LSK (Lin⁻Sca1⁺Kit⁺), HSC (Lin⁻Sca1⁺Kit⁺CD150⁺CD34⁻), CD150⁺CD34⁺ (Lin⁻Sca1⁺Kit⁺CD150⁺CD34⁺), CD150⁻CD34⁺ (Lin⁻Sca1⁺Kit⁺CD150⁻CD34⁺), and CD150⁻CD34⁻ (Lin⁻Sca1⁺Kit⁺CD150⁻CD34⁻). Bone marrow was harvested 24 hours after a single dose of LPS (0.5 mg/kg) or vehicle control. (B) Quantification of HSPC populations identified in (A) (percentage of live bone marrow cells). N = 4 to 6 per condition from three experiments. *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed unpaired Student’s t test.