Figure 2.

(A) Analysis of correlation between MELK expression and aneuploidy. MELK expression increases with higher aneuploidy levels in the TCGA breast cancer cohort. Aneuploidy is defined as the average DNA copy deviation from a euploid state. (B) Western blot of doxycycline-induced GFP-tagged MELK and endogenous MELK in RPE1 cells. Tubulin serves as a loading control. (C) Time-lapse imaging stills of mitotic RPE1 cells overexpressing GFP-tagged MELK showing that MELK localizes to the cleavage furrow during cytokinesis. Arrows indicate beginning (middle pane) and late cleavage furrow (right panel). (D) Frequency of mitotic abnormalities determined by time-lapse imaging (left panel) and quantification of karyotypes by metaphase spreads (right panel) in RPE1 cells with induced MELK overexpression. (E) Frequency of mitotic abnormalities determined by time-lapse imaging observed in MELK overexpressing RPE1 p53 knockout cells.