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. 2021 Sep 22;11(12):jkab335. doi: 10.1093/g3journal/jkab335

Figure 3.

Figure 3

(A) Western blots for MELK protein showing doxycycline-induced MELK knockdown in MCF10A, BT-20, MDA-MB-231, and RPE1 p53−/− cells compared to scrambled shRNA controls. Tubulin serves as a loading control. (B) Frequency of mitotic abnormalities determined by time-lapse imaging (left panel) and quantification of karyotypes by metaphase spreads (right panel) in MCF10A cells with MELK knockdown. (C–E) Frequency of mitotic abnormalities determined by time-lapse imaging observed in BT-20 cells (C), MDA-MB-231 cells (D), or RPE1 p53−/− cells with MELK knockdown (E).