Figure 4.

Filamentous growth of K. quercitrusa var. filamentosus (1120). Cells grown in the synthetic liquid medium 1% xylose (pH = 6) grow as filaments. Single yeast colonies from xylose agar plates were grown in 1% xylose liquid medium buffered at pH = 6 (see Methods). After 48–72 h at 30°C at 150 rpm, small cell chains are visible (A and B) elongating either from single cells (*) or a cluster of cells (**). Eventually, after 96–120 h, these structures develop to longer septate filaments (C and Ć) with bifurcations (arrowhead in Ć). Bright-field microscopy images at indicated scale.