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. 2021 Nov 26;12:788870. doi: 10.3389/fmicb.2021.788870

FIGURE 5.

FIGURE 5

HSP27 positively regulates the production of encephalomyocarditis virus (EMCV)-triggered IFN-I. (A) A549 cells were transfected with either 150 nmol of siHSP27-Mix or siNC for 36 h before infecting with EMCV at an multiplicity of infection (MOI) of 0.01 for 36 h. IFN-α and IFN-β mRNA levels were measured by RT-qPCR. Untreated group (UT) cells were used as control. (B) A549 cells were transfected with Myc-HSP27 (1 μg) or pCMV-Myc (1 μg) plasmids for 36 h before infecting with EMCV at an MOI of 0.01 for 36 h. IFN-α and IFN-β mRNA levels were measured by RT-qPCR. A549 cells transfected with pCMV-Myc (1 μg) plasmid were used as control. A549 cells were transfected with pCMV-HA (0.5 μg), HA-2C (0.5 μg) or HA-3A (0.5 μg) for 24 h, and then transfected with poly (I:C) for 8 h. (C) IFN-α mRNA levels were measured by RT-qPCR. (D) IFN-β mRNA levels were measured by RT-qPCR. Data were represented as mean ± SD of three independent experiments and were measured in technical duplicate, ***P < 0.001.