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. 2021 Nov 26;12:788870. doi: 10.3389/fmicb.2021.788870

TABLE 2.

Nucleotide sequences of the RT-qPCR primers.

Primer names Primer sequences (5′-3′)
Homo-HSP27-qF CTGACGGTCAAGACCAAGGATG
Homo-HSP27-qR GTGTATTTCCGCGTGAAGCACC
Homo-GAPDH-qF GTCTCCTCTGACTTCAACAGCG
Homo-GAPDH-qR ACCACCCTGTTGCTGTAGCCAA
Homo-IFN-α-qF GCCTCGCCCTTTGCTTTACT
Homo-IFN-α-qR CTGTGGGTCTCAGGGAGATCA′
Homo-IFN-β-qF TTGTTGAGAACCTCCTGGCT
Homo-IFN-β-qR TGACTATGGTCCAGGCACAG
EMCV-probe (FAM)CACTTCGATCACTATGCTTGCCGTT(Eclipse)
EMCV-3D-qF GTCATACTATCGTCCAGGGACTCTAT
EMCV-3D-qR CATCTGTACTCCACACTCTCGAATG