Fig. 3.

IgG antibody response in hamsters immunized with rBCG:TBDRchi evaluated by ELISA and immunoblot. a ELISA plates were coated with 1 µg of rTBDRchi. Sera from hamsters immunized with BCG Pasteur (negative control) and rBCG:TBDRchi collected at day 0 (pre-immune) and 51 (pre-challenge) were evaluated at 1:50 dilution, in triplicate, with an anti-hamster IgG used as secondary antibody. Results are presented as mean absorbance ± standard deviation. Significant differences were determined by one-way ANOVA, followed by Tukey multiple comparison. Asterisk indicates a significance difference (p < 0.05) to other groups. b Immunoblot with rTBDRchi, recombinant TBDR proteins and Leptospira lysate using sera obtained from hamsters after immunization with rBCG:TBDRchi (1:200). Humoral immune response was primarily direct against two of the leptospiral TBDRs, LIC10896 and LIC10964