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. 2021 Nov 9;99(12):skab336. doi: 10.1093/jas/skab336

Table 4.

Average of feed intake (g/d) body weight (kg), nitrogen intake (mg/BWkg0.75/d), nitrogen excretion (mg/BWkg0.75/d), and nitrogen deposition (mg/BWkg0.75/d) obtained in nitrogen balance trials for growing pigs challenged with E. coli lipopolysaccharide1

Nitrogen levels, % N as fed basis
Variables measured 0.94 1.69 2.09 3.04 3.23 3.97 Average SEM Linear Quadratic
Feed Intake1
 Control 411.9 417.2 419.1 418.8 413.3 426.6 417.8 9.70 0.532 0.917
 Challenged 373.5 375.0 384.5 370.6 362.7 371.6 373.0 9.70 0.533 0.754
Body Weight 2
 Control 19.6 19.3 18.7 18.7 18.4 19.2 19.0 0.66 0.409 0.272
 Challenged 19.4 19.0 18.8 18.7 18.7 19.1 18.9 0.66 0.272 0.661
Nitrogen Intake 3,4
 Control 519.7 958.5 1,216.0 1,773.3 1,883.0 2,309.7 1,443.3 24.82 <0.001 0.029
 Challenged 473.2 871.2 1,111.0 1,569.7 1,628.5 1,976.0 1,271.6 24.82 <0.001 0.001
P-value 0.306 0.057 0.023 <0.001 <0.001 <0.001
Nitrogen Excretion 3,4
 Control 184.1 309.3 407.3 545.9 483.9 783.2 452.3 36.23 <0.001 0.683
 Challenged 246.0 369.5 478.2 626.7 848.6 884.9 575.6 36.23 <0.001 0.474
P-value 0.249 0.262 0.188 0.135 <0.001 0.061
Nitrogen Deposition 3,4
 Control 335.6 649.2 808.6 1,227.3 1,405.9 1,526.5 992.2 42.13 <0.001 0.028
 Challenged 227.2 501.7 632.8 943.1 771.4 1091.1 694.6 42.13 <0.001 <0.001
P_value 0.079 0.018 0.005 <0.001 <0.001 <0.001

1Effect of challenge (P < 0.01), E. coli lipopolysaccharide (LPS) was used to induce inflammatory response in challenged group. The challenge consisted of an initial LPS dose of 30 μg/kg IM and subsequent dose of 33.6 μg/kg IM after 48 h. The control group received saline solution (0.9%, IM).

2Body weightbefore samplingperiod.

3Effect of N levels × challenge status interaction (P < 0.05).

4Polynomial contrasts: Linear effect of N levels (P < 0.01): NI, control NI = 215.13 + 350.91 NL and challenged NI = 246.87 + 292.80 NL (mg/BWkg0.75/d); NEX, control NEX = 86.27 + 104.54 NL and challenged NEX = 97.27 + 136.69 NL (mg/BWkg0.75/d); ND, control ND = 127.86 + 246.95 NL and challenged ND = 150.66 + 155.40 NL (mg/BWkg0.75/d).