Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Dec 10;12:7186. doi: 10.1038/s41467-021-27319-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — A Intestines from E15.5 control (Myh11^Cre-EGFP and R26^DTA176/+) and Myh11^Cre-EGFP R26^DTA176/+ embryos. Scale bar, 1 mm. The length of the small intestine was measured from the end of the stomach (orange arrowheads) to the beginning of cecum (purple arrowheads). B Lengths of E15.5 control (Myh11^Cre-EGFP n = 4 and R26^DTA176/+ n = 4) and Myh11^Cre-EGFP R26^DTA176/+ (n = 4) small intestines. Each point in the scatter plots represents the value from an individual embryo. Values are presented as means ± SD. *** adjusted P = 0.0007 by ordinary one-way ANOVA Tukey’s multiple comparisons test. C Schematic of method for measuring the opening angle to assess residual stress. We isolated approximately 1 mm long sections of E13.5 small intestine, cut the intestinal wall axially and measured the opening angle (θ), with the vertex at the midpoint of the inner epithelial surface and the rays intersecting the innermost edges of the cut wall. D The opening angles of intestinal segments from E13.5 control (wild type, n = 3, Myh11^Cre-EGFP, n = 3 and R26^DTA176/WT, n = 4) and Myh11^Cre-EGFP R26^DTA176/+ (n = 7) embryos. Adjusted P values are 0.0018 for Myh11^Cre-EGFP R26^DTA176/+ vs WT, 0.0054 for Myh11^Cre-EGFP R26^DTA176/+ vs Myh11^Cre-EGFP, and 0.005 for Myh11^Cre-EGFP R26^DTA176/+ vs R26^DTA176/WT. E The opening angles of intestinal segments from E13.5 Cilk1^+/- (n = 4) and Cilk1^−/− (n = 4) embryos. *p = 0.0138 by two-sided unpaired t test. F The opening angles of intestinal segments from E13.5 (Smo^lox/+, n = 6 and Dermo1^Cre Smo^lox/+, n = 4) and Dermo1^Cre Smo^lox/lox (n = 4) embryos. Adjusted P values are 0.0038 for Dermo1^Cre Smo^lox/lox vs Smo^lox/+ and 0.0058 for Dermo1^Cre Smo^lox/lox vs Dermo1^Cre Smo^lox/+. G Representative E13.5 wild-type intestine segments opened axially (WT + cut) or not before (Day 0) and after culture in Matrigel for 2 days (Day 2). Scale bar, 1 mm. H Length fold change of the cultured intestine segments (WT: n = 3; WT + cut: n = 3) for indicated days. D–F For each intestine, we calculated the median angle of 5–15 segments. D–F and H values are presented as means ± SD. *p < 0.05; **p < 0.01; ***p < 0.001 by ordinary one-way ANOVA Sidak’s multiple comparisons test, except in E. Source data are provided in the Source Data file.