Table 7.
Composition of PCR mix.
| Component | Volume per 10 μl reaction |
|---|---|
| SsoAdvanced™ Universal SYBR® Green Supermix | 5 μl |
| Foward and revers primers | 1 μl (0.8 μM) |
| DNA template | 2 μl (0.04–0.015 × 10–4) |
| Nuclease—free water | 2 μl |
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