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. 2021 Dec 10;11:23796. doi: 10.1038/s41598-021-03142-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of the ethanolic extract of D. esculentum on climbing ability of flies expressing Aβ42, (A–C) flies expressing Aβ42 (chr.2), and (D–F) flies expressing Aβ42 (chr.3). AD-free flies (elav-Gal4) were cultured on a normal medium, while AD flies (Aβ42) were cultured on a medium containing deionized water (DI), 1% DMSO, 125 µg/mL and 250 µg/mL of D. esculentum and 10 µM donepezil for the indicated days and then assayed for climbing ability. Values are mean ± SD of three assays and statistical significance was analyzed against AD flies (DI) by one-way ANOVA followed by Tukey’s test. *, p < 0.05; **, p < 0.01; ***, p < 0.001.