Fig. 1. In vitro RNA-antisense purification-based discovery of protein interactors of the SARS-CoV-2 –1PRF element.

A Schematic representation of the relevant genomic segment of SARS-CoV-2 as well as the location of the –1PRF element. B Schematic of in vitro interactome capture of protein interactors of the SARS-CoV-2 –1PRF element. In vitro synthesized RNA fragment numbered 1–84 corresponding to nucleotides 13456–13570 of the SARS-CoV-2 genome, was incubated with lysates of naïve HEK293 cells as well as SARS-CoV-2-infected and uninfected Calu-3 cells. The –1PRF RNA was captured by a biotinylated antisense DNA oligo and isolated proteins were subjected to LC-MS/MS. C Representative scatter plot of log₂-ratios comparing proteins captured in uninfected vs. SARS-CoV-2-infected Calu-3 cells. Core interactors common between uninfected and SARS-CoV-2-infected Calu-3 cells as well as uninfected HEK293 cells are highlighted in blue, ZAP is highlighted in pink. D Heatmap representing the enrichment (log₂) of core interactors. See also Supplementary Fig. 1D.