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. 2021 Dec 10;7:113. doi: 10.1038/s41531-021-00258-8

Fig. 3. PINK1 variants with disrupted mitophagy affect Parkin translocation.

Fig. 3

a Representative fluorescence images of HeLa PINK1 KO cells stably expressing EGFP-Parkin transfected with PINK1-HA WT and PINK1-HA variants p.Gly309Asp and p.Leu347Pro and before or after treatment with CCCP showing examples of three types of Parkin localization: clustered perinuclear aggregates, punctate diffuse aggregates, and diffuse cytosolic localization. Upper panels show EGFP-Parkin, middle panels show PINK1, and lower panels show an overlay including DAPI to stain nuclei. Scale bar, 10 μm. b Quantification of Parkin translocation by PINK1 WT or PINK1 missense variants upon CCCP treatment. Mean values are shown from three independent experiments. Bars are color-coded by the annotation as determined by Sherloc. Data analyzed using one-way ANOVA. *p < 0.05, **p < 0.005. c Quantification of variants for each of the types of Parkin localization, color-coded by the Sherloc annotation.