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. 2021 Dec 10;11:23820. doi: 10.1038/s41598-021-03339-z

Figure 3.

Figure 3

Morphological changes induced by PTN-PDT in LM2 cells and intracellular PTN localization. (A) LM2 cells were exposed 1 h to 30 µM PTN and immediately illuminated with 1.78 J/cm2. Cells were fixed after overnight incubation and stained by crystal violet for morphology observation. Micrographs were taken employing × 40 magnification. (B) LM2 cells were exposed 1 h to 30 µM PTN and fluorescence was documented at × 20 and × 40 (inset) magnification. Arrows indicate orange granules of PTN. Representative images are shown.