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. 2021 Dec 10;11:23820. doi: 10.1038/s41598-021-03339-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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PTN penetration into the tumour. SOT was topically applied with 1.96 mg of PTN in DMSO. After 2 h, tumours were excised, and fluorescence of fresh sections was visualized by fluorescence microscopy. The basal superficial section of the skin (upper plane) and the deeper region (mid-plane) were documented at × 10. Controls treated with DMSO were included.