Table 2.
Parameters describing the interaction of CNDs and HSA.
| CND type | KD (μM) | Nmax | n | rh,0 (nm) | Δrh,max (nm) | 〈KD〉 (μM) | ΔKD〈KD〉–1 |
|---|---|---|---|---|---|---|---|
| R-CNDs | 22.7 ± 5.3 | 1.2 ± 0.3 | 1.2 ± 0.2 | 0.69 ± 0.05 | 2.4 ± 0.1 | 32.3 | −0.30 |
| S-CNDs | 39.9 ± 9.5 | 1.2 ± 0.3 | 1.2 ± 0.2 | 0.73 ± 0.04 | 2.3 ± 0.1 | +0.24 | |
| N-CNDs | 34.3 ± 10.4 | 2 ± 0.6 | 1.0 ± 0.1 | 0.73 ± 0.05 | 2.5 ± 0.1 | +0.06 |
Apparent dissociation constant KD, maximum number Nmax of HSA molecules adsorbed per CND, cooperativity parameter n, hydrodynamic radius rh,0 of the CNDs without exposure to HSA, and maximum increase of hydrodynamic radius Δrh,max of the CNDs upon saturation with HSA. The error is the standard error as obtained by fitting of the data with the Hill-Model. ΔKD(S-CNDs) 〈KD〉–1 − ΔKD(R-CNDs) 〈KD〉–1 = 0.24 − (−0.30) = 0.54. Data were recorded with batch #1.