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. 2021 Dec 10;4:1387. doi: 10.1038/s42003-021-02910-8

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© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2021

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Fig. 6 — An artificial elastin-like polypeptide functionalized with a sequence from laminin can be used to generate organoids from pancreatic ductal progenitor cells from adult mice. (a) The recombinant protein (named aECM-lam) incorporates an IKVAV-containing 18-amino acid sequence derived from α1 laminin. The aECM-scr is a scrambled sequence control for aECM-lam. (b) aECM-lam permits the differentiation of endocrine (expressing C-peptide and glucagon) and acinar cell lineages (expressing amylase). (c) Individual organoids (Endocrine/Acinar) grown in aECM-lam express beta-cell maturation markers glucokinase, Pcsk1, and Pcsk2. (d) Organoids grown in aECM-lam are capable of secreting insulin in vitro when challenged by high concentrations of d-glucose or a combination of d-glucose and cAMP activator theophylline. The image is from ref. ¹⁹² and is reproduced with permission.