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. 2021 Dec 10;4:1384. doi: 10.1038/s42003-021-02914-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 10 — a Representative plot of isolated CECs differentiated from PBMCs. b, c Proliferation of CTV-labeled CD4⁺ (b) and CD8⁺ (c) T cells triggered by αCD3/αCD28 co-cultured with CECs differentiated from PBMCs (n = 4). P values were calculated with one-way ANOVA with Dunnett’s post hoc test. d Representative density plots of CEC differentiation from PBMCs based on CD71 and CD235a expression. e Proliferation of CTV-labeled CD4⁺ triggered by αCD3/αCD28 co-cultured with CEC-PBMCs at different developmental stages at 1:4 ratio. f, g Relative proliferation of CD4⁺ (f) and CD8⁺ (g) T cells co-cultured with CEC-PBMCs at different time points at a 1:4 ratio. P values were calculated with one-way ANOVA with Bonferroni’s post hoc test. h–j Levels of CD71 (h), CD235a (i), and CD49d (j) during erythroid differentiation from PBMCs. Data show means ± SD. Each point in b, c, f–j represents data from individual patients. n values are the numbers of individual patients used to obtain the data or the number of biological replicates in in vitro experiments. The source data underlying b, c, f–j are provided as a Supplementary Data 2 file.