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. 2021 Dec 10;4:1384. doi: 10.1038/s42003-021-02914-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Schematic presentation of the experimental setting. T cells isolated from OT-I mice were labeled with CellTraceViolet (CTV) and adoptively transferred to anemic and healthy control mice and stimulated with OVA. Scheme created using BioRender.com. b Percentage of proliferating (CTV^low) OT-I T cells in the spleen of NHA mice (n = 8), HA-PHZ mice (n = 8), and healthy controls (n = 5). Histograms show the fluorescence of CTV (CellTraceViolet)-V450 of OT-I T cells. P values were calculated with one-way ANOVA with Tukey’s post hoc test. c Representative plot of CD71 and TER119 levels in isolated CECs. Additional plots are presented in Supplementary Fig. 4. d Proliferation triggered by αCD3/αCD28 in CTV-labeled CD4⁺ T cells co-cultured with CECs isolated from the spleens of NHA (n = 8), HA-PHZ (n = 8), or HA-TER119 (n = 4) mice. T cell:CECs ratio was 1:2. Representative proliferation histograms of αCD3/αCD28-stimulated CD4⁺ T cells co-cultured with CECs. Histograms show the fluorescence of CTV (CellTraceViolet)-V450. P values were calculated with one-way ANOVA with Dunnet’s post hoc test. Data show means ± SD. Each point in b, d represents data from individual mice. n values are the numbers of mice. The source data underlying b, d are provided as a Supplementary Data 2 file.