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. Author manuscript; available in PMC: 2022 Dec 6.
Published in final edited form as: Curr Biol. 2021 Sep 2;31(23):5341–5349.e4. doi: 10.1016/j.cub.2021.08.022

Figure 2. Spatiotemporal and network properties of a single PaSNA episode.

Figure 2.

(A) Image frames during the first episode of PaSNA in an embryo. Phases labeled on top. Images are maximum intensity projections from an embryonic VNC expressing pan-neuronal GCaMP6s. Time stamps are relative to the positive inflection point caused by the activity burst. Yellow line delineates the VNC, with the ROI used for Panel B. Scale bar: 50 μm. (B) ΔF/F trace of the entire VNC during the first episode of PaSNA (n = 8). (C) ΔF/F of the color-coded four ROIs. Left displays −200 seconds to 600 seconds; right displays from −20 to 100 seconds relative to the initiation of PaSNA. (D-F’) Temporal projections (top) and ΔF/F VNC traces (bottom) for 30 seconds near the localized initiation time of the episode for control embryos (n = 8) (D), embryos expressing Kir2.1 pan-neuronally (E) (n = 5) and embryos expressing TNT pan-neuronally (F) (n = 6). (G) Schematic of Drosophila larval locomotor development showing activity at the muscle (top) and neuronal level (bottom). For all time series, dark lines represent the mean, while shading depicts the 95%CI. See also Video S2, S3, S4. For genotype information see Table S1.