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MS conditions for ivacaftor and colistin (polymyxin B is an internal standard for colistin).
| Compound Name | Quantitative Transition (m/z) | Qualitative Transition (m/z) | Retention Time (min) | Fragmentor (V) | Collision Energy (V) |
|---|---|---|---|---|---|
| Polymyxin B1 | 602.3 - 101.1 | 602.3 - 241.1 | 0.631 | 135 | 20 |
| Polymyxin B2 | 595.4 - 101.1 | 595.4 - 227.2 | 0.629 | 135 | 20 |
| Colistin A | 585.5 - 101.1 | 585.5 - 241.1 | 0.630 | 135 | 20 |
| Colistin B | 578.5 - 101.1 | 578.5 - 227.2 | 0.627 | 135 | 20 |
| Ivacaftor | 393.2 - 337.2 | 393.2 - 172.1 | 4.728 | 80 | 8 |
