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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: Adv Healthc Mater. 2021 Oct 8;10(23):e2101467. doi: 10.1002/adhm.202101467

Figure 4. IWR1 and IWP2 induce a concomitant increase in BMP4 expression and Smad1/5 phosphorylation on stiffer MC-GAG materials.

Figure 4.

Representative image (A) or quantitative densitometric analyses of Western blots (B-E) for phosphorylated Smad1/5 (p-Smad1/5), total Smad5, phosphorylated ERK1/2 (p-ERK1/2), total ERK1/2, phosphorylated Akt (p-Akt), Akt, Runx2, and GAPDH. QPCR of primary hMSCs cultured on NX-MC or MC for 7 days without (control) or with 50 μM IWR1 or IWP2 for (F) BMP2, (G) BMP2, and (H) BMP7 (n=3). Bars represent means, errors bars represent SE. Significant posthoc comparisons following ANOVA indicated with p values.