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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: Adv Healthc Mater. 2021 Oct 8;10(23):e2101467. doi: 10.1002/adhm.202101467

Figure 6. β-catenin knockdown regulates mechanotransduction mediators and osteogenic gene expression in a stiffness dependent manner.

Figure 6.

QPCR of primary hMSCs transfected with small interfering RNA targeting CTNNB1 (siCTNNB) or scrambled control (siControl) and cultured on NX-MC or MC for 7 days for (A) CTNNB1, (G) ALP, (H) COL1A1, (I) BSP2, and (J) RUNX2 (n=3). (B) Representative images and (C-F) quantification of Western blots of primary hMSCs transfected with siCTNNB or siControl and cultured on NX-MC or MC for 7 days for non-p-β-catenin, YAP, TAZ, p-FAK, FAK, and actin. Bars represent means, errors bars represent SE. Significant posthoc comparisons following ANOVA indicated with p values.