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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: Adv Healthc Mater. 2021 Oct 8;10(23):e2101467. doi: 10.1002/adhm.202101467

Figure 7. β-catenin knockdown increases mineralization on stiffer MC-GAG materials with a concomitant increase in BMPR signaling.

Figure 7.

(A) Representative images of Alizarin red staining of 4 micron sections of empty scaffolds (Empty) or hMSCs transfected with siCTNNB1 or siControl and differentiated on NX-MC or MC for 14 days (n=3). (C) Representative images of microCT of empty scaffolds (Empty) or hMSCs transfected with siCTNNB1 or siControl and differentiated on NX-MC or MC for 8 weeks (n=3). (B, D) Quantitative changes in mineralization in siCTNNB1 transfected cells on respective scaffolds expressed as percent mineralization in Alizarin red staining or microCT of siControl transfected cells. (E) Representative images and (F-I) quantification of Western blots of primary hMSCs transfected with siCTNNB or siControl and cultured on NX-MC or MC for 7 days for p-Smad1/5, Smad5, Runx2, p-ERK1/2, ERK1/2, p-Akt, and Akt. QPCR of primary hMSCs transfected with siCTNNB1 or siControl and cultured on NX-MC or MC for 7 days for (J) BMP2, (K) BMP4, and (L) BMP7 (n=3). Bars represent means, errors bars represent SE. Significant posthoc comparisons following ANOVA indicated with p values.